Article Sidebar

Download
PDF
Statistic
Read Counter : 106 Download : 13

Main Article Content

Abstract

Katokkon Chili is an important local variety in Mamasa, West Sulawesi, which frequently exhibits yellow mosaic and yellow curl symptoms. These symptoms are strongly suspected to be associated with viral infections, particularly those in the genera Begomovirus and Potyvirus. This study aimed to molecularly identify viruses causing yellow curl symptoms in Katokkon Chili plants. Symptomatic leaf samples were collected purposively from 14 locations in Mamasa Regency, West Sulawesi, followed by molecular detection and identification using universal Begomovirus and Potyvirus primers and sequencing analysis. Detection and identification results showed that chili plants yielded 912 bp DNA fragments with SPG1/SPG2 primers, confirming viral identity as Pepper Yellow Leaf Curl Indonesia Virus (PYLCIV) and demonstrating >98% homology with PYLCIV isolates from South Sulawesi. This study reports the first occurrence of PYLCIV in the Katokkon Chili variety in West Sulawesi and expands the host range of PYLCIV among chili varieties in Indonesia. These findings are important as a foundation for developing viral disease control strategies for chili plants in West Sulawesi.

Keywords

Begomovirus Host range Mamasa PCR Pepper yellow leaf curl Indonesia virus

Article Details

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

References

  1. Pasambe D, Kallo R. Industri hilir pengolahan cabai (Lada Katokkon) berbasis kelompok wanita tani. Bul Infbrmasi Teknol Pertan. 2017;(4):33–8.
  2. Maulana Z, Mentary N, Muhibuddin A. Morphological diversity characteristics of Chili Katokkon Capsicum cinenses Jacq. J Aquac Enviroment. 2023;5(2):84–8.
  3. Iriyani D, Nugrahani P. Kandungan klorofil, karotenoid, dan vitamin C beberapa jenis sayuran daun pada pertanian periurban di Kota Surabaya. J Mat Sains, dan Teknol. 2014;15(2):84–90.
  4. Flowrenzhy D, Harijati N. Pertumbuhan dan produktivitas tanaman Cabai Katokkon (Capsicum chinense Jacq.) di ketinggian 600 meter dan 1.200 meter di atas permukaan laut. J Biotropika. 2017;5(2):44–53.
  5. Sirappa MP, Heryanto R, Husnah N. Management of genetic resources in mamasa district: local chili characteristics of Lippak-Lippak. J Ilm Maju. 2019;2(2):25–32.
  6. Bandaso TS, Rauf A, Madauna IS. Response of Katokkon Chili lant (Capsium chinens Jacq.) to organic fertilizer. J Agrotekbis. 2022;10(4):508–20.
  7. Putri KS, Haerani N, Giono BRW. Growth of Katokkon Chili (Capsicum sinence Jacq) in the lowlands on application of organic mulch and banana weevil mol. J Agrotan. 2024;10(2):38–42.
  8. BPS-Statistics Sulawesi Barat Province. Produksi tanaman buah-buahan dan sayuran tahunan menurut kabupaten/kota dan jenis tanaman di Provinsi Sulawesi Barat, 2024 [Internet]. BPS-Statistics Indonesia, Agricultural Statistic for Horticulture SPH. 2025 [cited 2025 Aug 20]. Available from: https://sulbar.bps.go.id/id/statistics-table/3/U0dKc1owczVSalJ5VFdOMWVETnlVRVJ6YlRJMFp6MDkjMw==/produksi-tanaman-buah-buahan-dan-sayuran-tahunan-menurut-kabupaten-kota-dan-jenis-tanaman-di-provinsi-sulawesi-barat--2022.html
  9. Azzahra F, Munif A, Triwidodo H. Inventarisasi hama dan penyakit tanaman Cabai Katokkon (Capsicum chinense Jacq.) di Desa Ciapus, Kecamatan Ciomas, Kabupaten Bogor. IPB University; 2023.
  10. Marianah L. Insect vector and virus disease intensity on red chili plants. AgriHumanis J Agric Hum Resour Dev Stud. 2020;1(2):127–34.
  11. Suwardani NW, Purnomowati, Sucianto ET. Kajian penyakit yang disebabkan oleh cendawan pada tanaman cabai merah (Capsicum annum L.) di pertanaman rakyat Kabupaten Brebes. Scr Biol. 2014;3(3):223–6.
  12. Adiwena M, Siswanto A, Ngau M. Cendawan patogen pada tanaman cabai besar di wilayah perbatasan. J Ilm Respati. 2022;13(2):126–32.
  13. Ashwathappa K V., Reddy MK, Naik MK, Venkataravanappa V, Aswathanarayana DS, Govindappa MR, et al. Estimation of yield loss due to chilli leaf curl disease in chilli (Capsicum annuum L.) at different stages of the crop. Pest Manag Hortic Ecosyst. 2022;28(1):147–51.
  14. Nalla MK, Scha R, Pappu HR, Barchenger DW. Current status, breeding strategies and future prospects for managing chilli leaf curl virus disease and associated begomoviruses in Chilli (Capsicum spp.). Front Plant Sci. 2023;14:1223982.
  15. Widodo CJ, Taufik M, Khaeruni A, Mallarangeng R. Determination of Begomovirus on chili plants (Capsicum sp.) in Buton and Muna Islands, Southeast Sulawesi, Indonesia. Biodiversitas J Biol Divers. 2023;24(3):741–51.
  16. Thakur H, Jindal SK, Sharma A, Dhaliwal MS. Chilli leaf curl virus disease: a serious threat for chilli cultivation. J Plant Dis Prot. 2018;125:239–249.
  17. Sulandari S, Suseno R, Hidayat SH, Harjosudarmo J, Sosromarsono S. Detection and host range study of virus associated with pepper yellow leaf curl disease. HAYATI J Biosci. 2006;13(1):1–6.
  18. Adediji AO, Fakolujo TI, Atiri GI. Occurrence and molecular characterization of Pepper veinal mottle virus from solanaceous crops. Int J Veg Sci. 2020;27(2):187–97.
  19. Akhter MS, Akanda AM, Kobayashi K, Jain RK, Mandal B. Plant virus diseases and their management in Bangladesh. Crop Prot. 2019;118:57–65.
  20. Sutrawati M, Djamilah, Inata A. Infection of Cucumber mosaic virus and Chilli veinal mottle virus on Chillipepper in Rejang Lebong Regency, Bengkulu. J Fitopatol Indones. 2012;8(4):110–5.
  21. Ashfaq M, Ahmad A. First report of Tomato spotted wilt virus in hot pepper in Pakistan. J Plant Pathol. 2017;99(1):291.
  22. Selangga DGW, Listihani L, Temaja IGRM, Wirya GNAS, Sudiarta IP, Yuliadhi KA. Determinants of symptom variation of Pepper yellow leaf curl Indonesia virus in bell pepper and its spread by Bemisia tabaci. Biodiversitas J Biol Divers. 2023;24(3).
  23. Nyana IDN, T MTL, Adnyani NNP, Suastika G. Identification of Pepper vein yellows virus Associated with Yellow Vein Banding Disease on cucumber plant in Bali. J Fitopatol Indones. 2016;12(4):109–16.
  24. Listihani, Selangga DGW. Molecular identification of sweet potato virus C on sweetpotato in Bali, Indonesia. J Perlindungan Tanam Indones. 2021;25(1):56–63.
  25. Setiawati W, Udiarto BK, Soetiarso TA. Pengaruh varietas dan sistem tanam cabai merah terhadap penekanan populasi hama kutu kebul. J Hortik. 2008;18(1):55–61.
  26. Kasim NN, Nasaruddin A, Melina. Identifikasi thrips (Thysanoptera) pada tanaman tomat dan cabai di tiga kabupaten. J TABARO. 2017;1(1):67–77.
  27. Wahyono A, Murti RH, Hartono S, Nuringtyas TR, Wijonarko A, Mulyantoro M, et al. Current status and complexity of three Begomovirus species in pepper plants in lowlands and highlands in Java Island, Indonesia. Viruses. 2023;15:1278.
  28. Duriat AS, Gunaeni N, Wulandari AW. Penyakit penting pada tanaman cabai dan pengendaliannya. Monografi. Balai Penelitian Tanaman Sayuran; 2007.
  29. Koeda S, Kesumawati E, Tanaka Y, Hosokawa M, Doi M, Kitajima A. Mixed infection of Begomoviruses on pepper plants at northern Sumatra, Indonesia. Trop Agric Dev. 2016;60(2):59–64.
  30. Koeda S, Homma K, Mari Kamitani, Nagano AJ, Taniguchi M, Pohan N, et al. Pepper vein yellows virus 9: a novel polerovirus isolated from chili pepper in Indonesia. Arch Virol. 2020;165:3017–3021.
  31. Annisaa NW, Hidayat P, Giyanto, Hidayat SH, Lee S. Multiple infections of Begomovirus on its host plants. In: International e-Conference on Sustainable Agriculture and Farming System. Bogor: IOP Publishing; 2021. p. 012047.
  32. Hamdayanty, Hardina N. Identification of virus that causes yellow leaf curl disease in chili in Gowa Regency, South Sulawesi. J Agrik. 2023;34(3):427–34.
  33. Doyle JJ, Doyle JL. A rapid DNA isolation of procedure for small quantities of fresh leaf tissue. Phytochem Bull. 1987;19(1):11–5.
  34. Ha C, Coombs S, Revill PA, Harding RM, Vu M, Dale JL. Design and application of two novel degenerate primer pairs for the detection and complete genomic characterization of potyviruses. Arch Virol. 2008;152:25–36.
  35. Li R, Salih S, Hurtt S. Detection of geminiviruses in sweetpotato by polymerase chain reaction. Plant Dis. 2004;88(12):1291–396.
  36. Wiridannisaa N, Kasim NN, Prihatin P. Identification of Begomoviruses causing yellow curl disease in chilli in West Java. J Biol Trop. 2025;25(3):4231–4238.
  37. Windarningsih M. Penyebaran penyakit virus daun menguning dan keriting pada cabai rawit di Kabupaten Lombok Utara. Crop AGRO Sci J Agron. 2018;11(2):145–152.
  38. Kushwaha JK, Rai AK, Srivastava D. Top ten viruses reported on chilly in Southeast Asia. Virol Mycol. 2021;10(3):209.
  39. Ahmad A, Tiberini A, Ashfaq M, Tomassoli L. First report of Pepper mild mottle virus infecting chilli pepper in Pakistan. New Dis Reports. 2015;32(1):1–36.
  40. Gadhave KR, Gautam S, Rasmussen DA, Srinivasan R. Aphid transmission of potyvirus: the largest plant-infecting rna virus genus. Viruses. 2020;12:773.
  41. Matthews REF. Fundamentals of plant virology. California: Academic Press is an Imprint of Elsevier; 1992.
  42. Sakata J ji, Shibuya Y, Sharma P, Ikegami M. Strains of a new bipartite begomovirus, pepper yellow leaf curl Indonesia virus, in leaf-curl-diseased tomato and yellow-vein-diseased ageratum in Indonesia. Arch Virol. 2008;153:2307–2313.
  43. Fang M, Yu J, Kim K hyung. Pepper mottle virus and its host interactions: current state of knowledge. Viruses. 2021;13:1930.
  44. Yoon Y joung, Venkatesh J, Lee J ho, Kim J, Lee H eun, Kim D sun, et al. Genome editing of eIF4E1 in tomato confers resistance to pepper mottle virus. Front Plant Sci. 2020;11:1098.
  45. Liu L, Venkatesh J, Jo YD, Koeda S, Hosokawa M, Kang JH, et al. Fine mapping and identification of candidate genes for the sy-2 locus in a temperature-sensitive chili pepper (Capsicum chinense). Theor Appl Genet. 2016;129:1541–1556.
  46. Hasan A, Taufik M, Gusnawaty HS, Sarawa. Uji kisaran inang Potyvirus penyebab mosaik nilam (Pogostemon cablin (Blanco) Benth) asal Sulawesi Tenggara. J Agroteknos. 2014;4(3):194–201.
  47. Minicka J, Taberska A, Borodynko-Filas N, Kaźmińska K, Bartoszewski G, Hasiów-Jaroszewska B. Viruses infecting Capsicum crops in Poland and molecular characterization of newly detected bell pepper alphaendornavirus (BPEV). Crop Prot. 2024;176:106478.
  48. Fauquet CM, Stanley J. Geminivirus classification and nomenclature: progress and problems. Ann Appl Biol. 2003;142(2):165–89.