Perancangan primer gen lktB pada Fusobacterium necrophorum untuk analisis PCR

Primer design of lktB gene on Fusobacterium necrophorum for PCR assay

Authors

  • Rian Ka Praja Biomedical Sciences Program, Faculty of Medicine, Udayana University Denpasar, Bali, Indonesia, 80113
  • Reny Rosalina Chemistry Department, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jawa Barat, Indonesia, 40132

DOI:

https://doi.org/10.31605/jstp.v2i2.960

Keywords:

foot rot, Fusobacterium necrophorum, in silico, leukotoxin B, necrobacillosis, primer

Abstract

Fusobacterium necrophorum is a pathogen causing disease in animals, especially cattle, goats, and sheep. F. necrophorum infection can result in a variety of necrotic conditions (necrobacillosis). This study aimed to design a pair of primers for detecting the leukotoxin B (lktB) gene expressed by F. necrophorum as diagnostic support. The lktB gene sequence was obtained from GenBank NCBI with accession number AF312861.3:685-2337. Furthermore, the sequence was used as a template for in silico primer design using Primer-BLAST. Primer candidates successfully designed were then analyzed for their secondary structure using NetPrimer. The results showed that forward primer set 6 (5'-TCGGATGCTGGAATGCTACTT-3') and reverse primer set 6 (5'-GGGCTCCCAAATCCTTACGA-3') were a favorable primer set with a product size of 228 bp. However, laboratory experiments need to be carried out to determine the optimal conditions for this primer set.

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Published

23-06-2021

How to Cite

Praja, R. K., & Rosalina, R. (2021). Perancangan primer gen lktB pada Fusobacterium necrophorum untuk analisis PCR: Primer design of lktB gene on Fusobacterium necrophorum for PCR assay. Jurnal Sains Dan Teknologi Peternakan, 2(2), 47–55. https://doi.org/10.31605/jstp.v2i2.960

Issue

Vol. 2 No. 2 (2021): Jurnal Sains dan Teknologi Peternakan

Section

Articles

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