Utilization of Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract as an Antioxidant in Cow’s Milk Kefir

Pemanfaatan Ekstrak Kulit Buah Naga Merah (Hylocererus polyrhizus) sebagai Antioksidan pada Kefir Susu Sapi

Authors

  • Yola Desnera Putri Sekolah Tinggi Farmasi Indonesia
  • Restiani Novitasari Sekolah Tinggi Farmasi Indonesia
  • Sohadi Warya Sekolah Tinggi Farmasi Indonesia
  • Adit Andriyannto Sekolah Tinggi Farmasi Indonesia

Keywords:

Kefir, Dragon Fruit Peel Extract, Antioxidant, IC50

Abstract

Kefir, a fermented beverage rich in probiotics, exhibits antioxidant properties capable of neutralizing free radicals. To enhance its antioxidant activity, this study utilized extract from red dragon fruit peel (Hylocereus polyrhizus), which is abundant in polyphenols, as a natural antioxidant source. This extract is expected to improve the antioxidant efficacy of cow milk-based kefir. The research aimed to evaluate the impact of adding red dragon fruit peel extract on the antioxidant activity and chemical parameters of cow milk kefir. The methodology involved producing kefir with varying extract concentrations (0.002%, 0.1%, 0.2%), assessing antioxidant activity via the DPPH (free radical scavenging) method, and conducting chemical analyses including pH, protein content, fat content, and lactic acid levels. Results revealed that control kefir (no extract) had an IC₅₀ of 93.96 µg/mL, pure peel extract 19.96 µg/mL, kefir with 0.2% extract 29.61 µg/mL, 0.1% extract 37.28 µg/mL, and 0.002% extract 75.47 µg/mL. Extract supplementation also yielded pH 4.51, protein 3.84%, fat 5.67%, and lactic acid 1.24%. Overall, incorporating red dragon fruit peel extract successfully boosted the antioxidant potential of cow milk kefir while producing a product with chemical profiles meeting quality standards, positioning it as a promising functional food candidate

Author Biographies

Yola Desnera Putri, Sekolah Tinggi Farmasi Indonesia

Departemen Farmasetika, Sekolah Tinggi Farmasi Indonesia, Kota Bandung, Jawa barat 40266, Indonesia

Restiani Novitasari, Sekolah Tinggi Farmasi Indonesia

Departemen Farmasetika, Sekolah Tinggi Farmasi Indonesia, Kota Bandung, Jawa barat 40266, Indonesia

Sohadi Warya, Sekolah Tinggi Farmasi Indonesia

Departemen Farmasetika, Sekolah Tinggi Farmasi Indonesia, Kota Bandung, Jawa barat 40266, Indonesia

Adit Andriyannto, Sekolah Tinggi Farmasi Indonesia

Departemen Farmasetika, Sekolah Tinggi Farmasi Indonesia, Kota Bandung, Jawa barat 40266, Indonesia

References

Aiello, F., Restuccia, D., Spizzirri, U. G., Carullo, G., Leporini, M., & Loizzo, M. R. (2020). Improving kefir bioactive properties by functional enrichment with plant and agro-food waste extracts. Fermentation, 6(3), 83. https://doi.org/10.3390/fermentation6030083

Aliya, N., Riyanta, A. B., & Muldiyana, T. (2024). Uji aktivitas antioksidan ekstrak kulit dan daging buah naga merah (Hylocereus polyrhizus) dan penentuan parameter non spesifik. Jurnal Riset Kefarmasian Indonesia, 6(1), 1–15. https://doi.org/10.33759/jrki.v6i1.485

Devitria, R., Wulandari, R., & Elfia, M. (2023). Water soluble ash content and acid insoluble ash content test on guava seed simplicia (Syzygium malaccense). Jurnal Ilmu Kesehatan Abdurrab, 1(2), 12–16.

Fahiroh, J., Arifin, N., Devitri, R. W., Septiarini, R. T., Zahara, E. S., Maulidya, V., et al. (n.d.). Tinjauan literatur: Efektivitas maserasi sebagai metode ekstraksi fitokimia. https://doi.org/10.61132/obat.v3i6.19

Farag, M. A., Jomaa, S. A., Abd El-Wahed, A., & El-Seedi, H. R. (2020). The many faces of kefir fermented dairy products: Quality characteristics, flavour chemistry, nutritional value, health benefits, and safety. Nutrients, 12(2), 346. https://doi.org/10.3390/nu12020346

Friska Pertiwi, A., Taufik, E., & Arief, I. I. (2022). Karakteristik kefir susu sapi dengan penambahan ekstrak bunga telang (Clitoria ternatea). Jurnal Ilmu Pertanian Indonesia, 28(1), 34–45. https://doi.org/10.18343/jipi.28.1.34

Galingging, A., Ratnaningsih, A. T., & Lestari, I. (2022). Kunci determinasi famili Dipterocarpaceae di arboretum Universitas Lancang Kuning. Jurnal Penelitian Kehutanan Bonita, 4.

Handayani, F., Apriliana, A., Novianti, I., et al. (2020). Karakterisasi dan skrining fitokimia simplisia buah selutui puka (Tabernaemontana macracarpa Jack). Jurnal Ilmiah, 12(1).

Horwitz, W. (2006). Official methods of analysis of AOAC International. AOAC International.

Julianto, B., Rossi, E., et al. (2016). Karakteristik kimiawi dan mikrobiologi kefir susu sapi dengan penambahan susu kedelai. JOM Faperta, 3(1).

Marjenah, M., Kustiawan, W., Nurhiftiani, I., et al. (2018). Pemanfaatan limbah kulit buah-buahan sebagai bahan baku pembuatan pupuk organik cair. ULIN: Jurnal Hutan Tropis, 1(2).

Milanda, T., & Kusuma, S. A. F. (2025). Kefir: Minuman fermentasi dengan manfaat antibakteri. Jurnal Ilmiah Global Farmasi, 3(1).

Nwachukwu, I. D., Sarteshnizi, R. A., Udenigwe, C. C., & Aluko, R. E. (2021). A concise review of current in vitro chemical and cell-based antioxidant assay methods. Molecules, 26(16), 4865. https://doi.org/10.3390/molecules26164865

Plaskova, A., & Mlcek, J. (2023). New insights of the application of water or ethanol-water plant extract rich in active compounds in food. Frontiers in Nutrition, 10. https://doi.org/10.3389/fnut.2023.1118761

Puspita Sari, R., Laoli, M. T., et al. (2019). Karakterisasi simplisia dan skrining fitokimia serta analisis KLT daun dan kulit buah jeruk lemon (Citrus limon). Jurnal Farmasi, 2(2), 59–68.

Putri, Y., Setiani, N. A., & Ayuningtyas, Y. (2020). Standardisasi mikrobiologi kefir dari susu kambing dan susu sapi. Journal of Pharmacopolium, 3(2).

Putri, Y. D., Setiani, N. A., & Warya, S. (2020). The effect of temperature, incubation and storage time on lactic acid content, pH, and viscosity of goat milk kefir. Current Research on Biosciences and Biotechnology, 2(1), 101–104.

Sulistiawanti, N. L., Hairunnisa, H., Widya, N., & Fitriyanti, S. (2025). Perbedaan penggunaan pelarut terhadap nilai rendemen yang dihasilkan dengan berbagai metode ekstraksi dalam proses ekstraksi. An-Najat, 3(3), 213–231.

Ummy, M., Fadhilah, S., & Korry, N. (2025). Uji aktivitas antioksidan ekstrak kulit buah naga (Hylocereus monacanthus). Pharmacoscript, 8(2), 326–339. https://doi.org/10.36423/pharmacoscript.v8i2.2159

Vieira, C. P., Rosario, A. I. L. S., Lelis, C. A., et al. (2021). Bioactive compounds from kefir and their potential benefits on health: A systematic review and meta-analysis. Oxidative Medicine and Cellular Longevity, 2021. https://doi.org/10.1155/2021/9081738

Zakaria, Y., Delima, M., & Diana, E. (2013). Analisa keasaman dan total bakteri asam laktat yogurt akibat bahan baku dan persentase Lactobacillus casei yang berbeda. Agripet, 13(2).

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Published

2026-07-06

How to Cite

Putri, Y. D., Novitasari, R., Warya, S., & Andriyannto, A. (2026). Utilization of Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract as an Antioxidant in Cow’s Milk Kefir: Pemanfaatan Ekstrak Kulit Buah Naga Merah (Hylocererus polyrhizus) sebagai Antioksidan pada Kefir Susu Sapi. Nutrition Science and Health Research, 5(1), 1–13. Retrieved from https://ojs.unsulbar.ac.id/index.php/nutrition/article/view/6482